When the enzyme concentration remains fixed and the substrate amount is increased, why does the reaction rate eventually stop rising despite further substrate addition?

Biology Mcqs for PPSC, FPSC, NTS, and Pakistan government job tests. Select an option below, then read the explanation.

PPSCFPSCNTSPakistan govt jobs
Subject
Biology Mcqsbiology-mcqs
Published
11 May 2018
Last updated
28 May 2026

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Explanation

The reaction rate plateaus because all the enzyme's active sites are saturated with substrate molecules, preventing any further increase in reaction speed despite more substrate being available.

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